Primary Sexual Differences
Examining the shape of the genital papilla is the best and most efficient way to distinguish the sexes across all genera of the subfamily. After a specimen reaches maturity, the papilla forms a distinctive shape.
♂ Male
Abdomen of a male
The genital papilla of a male Loricariinae specimen is thin and pointed. In some species the plate-lacking area surrounding the papilla is shaped compressed.
♀ Female
Abdomen of a female
Female Loricariinae specimens have a thick and blunt genital papilla. The plate-lacking area surrounding the papilla can be shaped elongated in some species.
Secondary Sexual Differences
Breeding odontodes on headsides

One of the most known secondary difference of the sexes are the breeding odontodes of mature males.

♂ Male
Male
♀ Female
Female

According to the concrete species the breeding odontodes at the headsides of a mature male can differ in size and shape. In some species it is just developed during the breeding season, some other do not have a temporal restriction.
Attention: It is known that females of a few loricariinae species also can develop breeding odontondes at the headsides. That was proved for some female specimen of Sturisoma festivum and Sturisomatichthys tamanae.

Genera typically showing this characteristic
Breeding odontodes on pectoral fins

Next to the breeding odontodes on the headsides, mature males can develop also breeding odontodes on the pectoral fins.

♂ Male
Male
♀ Female
Female

There are 2 different types of the development of breeding odontodes on pectoral fins. The first one concerns just the pectoral spine. On that spine mature males of Lamontichthys, Harttia or Harttiella develop spiky odontodes. These odontodes often are just developed temporarily during the breeding season. The seconds type with a plenty of straight odontodes all over the fin is known from species of Rineloricaria senu lato.

Genera typically showing this characteristic
Thickened pectoral spines

The males of some few species have thicked pectoral spines.

♂ Male
Male
♀ Female
Female

In some loricariinae species with rarely secondary sexual differences the males have slightly thicked spines of the pectoral fins. This difference is visible after they got mature.

Genera typically showing this characteristic
Head width

Often the head of male loricariinae specimen seems to be broader than the head of females.

♂ Male
Male
♀ Female
Female

This characteristic is developed more or less in all species of the subfamily. Sometimes it is really conspicuous, but mostly it is attended by the breeding odontodes on the headsides of male specimen.

Genera typically showing this characteristic
Structure of the lips

Some lipbrooding species develop enlarged lower lips during breeding season.

♂ Male
Male
♀ Female
Female

The males of the lipbrooding species out of the Loricaricariithys-Group develop a remarkable enlarged lower lip during the breeding season. With that enlarged lipstructure the batch of eggs is enclosed as good as possible to carry until the fry are hatched.
The males of lipbrooding species from other evolutionary groups as Loricaria-Group or Pseudohemiodon-Group with fine branched lips don't show that remarkable enlargement of the lower lip.

Genera typically showing this characteristic